艾德思：抗生素抗性基因在土壤食物链中的营养转移

中国科学院城市环境研究所朱永官教授团队以土壤跳虫(Folsomia candida)-捕食性螨(Hypoaspis aculeifer)为模型食物链,研究了粪便污染土壤生态系统中ARGs的营养转移.相关成果发表于 Environmental Science & Technology (IF=).

Abstract

The presence and spread of antibiotic resistance genes (ARGs) are causing substantial global public concern; however, the dispersal of ARGs in the food chain is poorly understood. Here, we experimented with a soil collembolan (Folsomia candida)-predatory mite (Hypoaspis aculeifer) model food chain to study trophic transfer of ARGs in a manure-contaminated soil ecosystem. Our results showed that manure amendment of soil could significantly increase ARGs in the soil collembolan microbiome. With the ARGs in the prey collembolan microbiome increasing, an increase in ARGs in the predatory mite microbiome was also observed, especially for three high abundant ARGs (blaSHV, fosX and aph6ia). Three unique ARGs were transferred into the microbiome of the predatory mite from manure amended soil via the prey collembolan (aac(6′)-lb(akaaacA4), yidY_mdtL and tolC). Manure amendment altered the composition and structure and reduced the diversity of the microbiomes of the prey collembolan and the predatory mite. We further reveal that bacterial communities and mobile genetic elements were two important drivers for the trophic transfer of ARGs, not just for ARGs distribution in the samples. These findings suggest that the importance of food chain transmission of ARGs for the dispersal of resistance genes in soil ecosystems may be underestimated.

抗生素抗性基因(ARGs)的存在和传播引起了全球公众的广泛关注,然而,人们对ARGs在食物链中的传播知之甚少.本文以土壤跳虫(Folsomia candida)-捕食性螨(Hypoaspis aculeifer)为模型食物链,研究了粪便污染土壤生态系统中ARGs的营养转移.结果表明,土壤中加入粪便能显著提高土壤跳虫微生物群落中的ARGs含量.随着土壤跳虫微生物群中的ARGs含量的增加,捕食性螨微生物群中的ARGs含量也有所增加,尤其是三种丰度较高的ARGs( blaSHV / fosX 和 aph6ia ).三种独特的ARGs通过被捕食的跳虫 (aac(6′)-lb(akaaacA4) / yidY_mdtL 和 tolC ) ,从加入粪便的土壤中转移到捕食性螨的微生物群落中.粪便的加入改变了被捕食的跳虫和捕食性螨微生物群落的组成和结构,降低了其多样性.研究进一步发现,细菌群落和可移动遗传元件是ARGs随营养转移的两个重要驱动因素,而不仅仅是土壤中的ARGs分布.这些发现表明,在土壤生态系统中,ARGs随食物链的传播对抗生素抗性基因扩散的重要性可能被低估.

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